What cryobags can be used in the VIA Thaw CB1000?
What volume can I fill the cryobags to?
As specified by the cryobag manufacturer, see Cryobags compatible with the VIA Thaw CB1000. The VIA Thaw CB1000 has been tested with fill volumes between 10 and 275ml. For fill volumes outside of this, contact Asymptote.
How does the VIA Thaw for Cryobags work?
The thawing system works by warming cryobags on a conformable precision-machined aluminium bed. This gives good heat transfer as a result of the beds shape conforming to variations in bag shape. Sophisticated control systems monitor six different temperature sensors hundreds of times a second to control the thaw. Once the contents of the cryobag are sufficiently thawed the agitators start gently prodding the bag mixing the contents of the cryobag, averaging out temperatures.
How does the VIA Thaw CB1000 detect when thawing is complete?
The system measures the surface temperature of the cryobag in several places. The onboard software processes these measurements to determine when thawing is complete.
How can I be sure my sample has correctly thawed?
The VIA Thaw has been designed to warm Cryobags from liquid nitrogen or dry-ice storage to the instant that the ice has fully thawed. By ensuring the thawed liquid temperature is a low as a possible, the damaging effects of DMSO-based cryoprotectants at elevated temperatures can be minimised. The system may eject the cryobag with small ice-crystals still briefly visible. This should disappear within 20 seconds of gentle agitation. If no ice crystal is observed, this should be acceptable as the long as the thaw time is within the normal range for this cryobag and fill combination, and the system has not reported any warnings. If the crystal takes more than 20 seconds to disappear, continue to agitate gently until it has completely melted.
Can the environment affect thawing?
The VIA Thaw CB1000 is designed to work in ambient temperatures from +5°C to +30°C and is not affected by normal air flow e.g. from working in a hood.
Can the VIA Thaw CB1000 be used in a GMP environment?
The VIA Thaw CB1000 has been designed to be ready for a GMP environment, but it is the responsibility of the user to appropriately qualify the system within their particular process and to obtain any necessary certifications as required by their local and national regulations.
What are thawing protocols?
Please see our guide to understanding thawing protocols.
What do I do if a cryobag is ejected too cold?
Repeat the thaw. Before repeating the thaw ensure that:
· Any overwraps are removed
· Any slush is manually mixed
· That the correct profile is chosen
· Ensure that the bag is positioned correctly
· If the bag is still solid and quite deformed, ensure that the thickest part of the bag is positioned underneath the IR sensors
· Ensure that the lid closes